SubtiBank SubtiBank
spoIIIE [2018-02-16 08:55:28]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

spoIIIE [2018-02-16 08:55:28]

ATP-dependent dsDNA translocase, resolution of chromosomal dimers after DNA replication, transports the forespore chromosome across the sporulation septum
Locus
BSU16800
Isoelectric point
6.76
Molecular weight
86.96 kDa
Protein length
787 aa Sequence Blast
Gene length
2361 bp Sequence Blast
Function
chromosome partition during sporulation
Product
ATP-dependent dsDNA translocase required for chromosome partition
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Genetics → DNA condensation/ segregation
  • Lifestyles → Sporulation → Sporulation/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,752,272 → 1,754,641

    The protein

    Catalyzed reaction/ biological activity

  • transports the forespore chromosome across the sporulation septum PubMed
  • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
  • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed
  • binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG PubMed

    • Paralogous protein(s)

  • SftA

    • Domains

  • N-terminal transmembrane domain (4 helices) PubMed
  • 134 aa linker PubMed
  • 512 aa motor domain at the C-terminus PubMed

    • Structure

  • 2IUT (FtsK from Pseudomonas aeruginosa, partial, 48% identity, complex with SftA) PubMed

    • Localization

  • transmembrane domain mediates dynamic localization to active division sites PubMed
  • complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of sporulation and division septa PubMed

    • Expression and Regulation

    Operons

    Genes
    spoIIIE-ymfC
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
    • view in new tab

    Biological materials

    Mutant

  • BKE16800 (ΔspoIIIE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
  • BKK16800 (ΔspoIIIE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT

    • References

    Reviews

    Loading

    Original publications

    Loading